Repeated cycles of amplification cause degradation of your probe, causing improved fluorescence. TaqMan detection is, for that reason, extra specific than dye‐dependent detection, because a rise in fluorescence takes place only on binding in the probe to a particular sequence and subsequent de‐quenching, although no signal is produced by amplification https://www.facebook.com/NewTechFind/posts/pfbid0gFLrpEPW4jhtWM7JBsKbBkAth2gALUGDD93VCacGPjRKPiAeKBr87g5EJv9Tk4Qdl